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dulbecco s phosphate buffered saline dpbs  (Thermo Fisher)


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    Thermo Fisher dulbecco s phosphate buffered saline dpbs
    Dulbecco S Phosphate Buffered Saline Dpbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphate+buffered+saline+dpbs/Phosphate-buffered+saline+(DPBS%2C+1X)%2C+Dulbecco's+formula/pmc13241941-84-15-24
    Average 99 stars, based on 1 article reviews
    dulbecco s phosphate buffered saline dpbs - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Commercially available acellular matrices for adipose-derived stem cell-based tissue engineering: a comparative analysis of morphology and early cytocompatibility
    Article Snippet: .. The isolation of adipose-derived stem cells (ASCs) was performed using 0.2% collagenase type II (Worthington Biochemical Corp., Lakewood, USA) dissolved in Dulbecco’s Phosphate Buffered Saline (DPBS) (Gibco, Thermo Fisher Scientific, Massachusetts, USA). ..

    Saline:

    Article Title: Commercially available acellular matrices for adipose-derived stem cell-based tissue engineering: a comparative analysis of morphology and early cytocompatibility
    Article Snippet: .. The isolation of adipose-derived stem cells (ASCs) was performed using 0.2% collagenase type II (Worthington Biochemical Corp., Lakewood, USA) dissolved in Dulbecco’s Phosphate Buffered Saline (DPBS) (Gibco, Thermo Fisher Scientific, Massachusetts, USA). ..

    Article Title: Characterization of genipin-crosslinked gelatin/PVA hydrogels and the chondroprotective influence of hydroxytyrosol: an In vitro study.
    Article Snippet: .. The harvested tissue was thoroughly rinsed with Dulbecco’s Phosphate-Buffered Saline (DPBS) (Gibco, Carlsbad, CA, USA) supplemented with 1% of antibiotic-antimycotic solution (AA; Gibco, Weilam Rhein, Germany). .. The specimens were then minced into small fragments (1–2 mm3) and subjected to enzymatic digestion using 0.6% Collagenase Type II (Worthington Biochemical Corp., NJ, USA).

    Article Title: Ice Templated PEG-Alginate Double-Network Cryogels with Tunable Mechanics and Degradation for Soft Tissue Engineering.
    Article Snippet: 8-arm polyethylene glycol acrylate (8-arm PEG acrylate, 10 kDa; Jenkem Technology, Plano, TX, USA), sodium alginate with an M/G ratio of 0.6 (Protanal® LF 10/60, IFF Pharma Solutions, Wilmington, DE, USA), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC; Thermo Fisher Scientific, Waltham, MA, USA), N-hydroxysuccinimide (NHS; Tokyo Chemical Industry, Tokyo, Japan), 2-(N-morpholino) ethanesulfonic acid buffer (MES, 1.0 M, pH 7.0; Thermo Fisher Scientific), and adipic acid dihydrazide (AAD; Tokyo Chemical Industry). .. Dithiothreitol (DTT) and ethylene glycol bis(mercaptoacetate) (EGBMA), Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12, 1:1), fetal bovine serum (FBS), penicillin–streptomycin (PS), L-glutamine, Dulbecco’s phosphate-buffered saline (DPBS), and 0.05% trypsin–EDTA, Rat tail collagen type I, The LIVE/DEAD Viability/Cytotoxicity Kit, 4′,6-diamidino-2-phenylindole (DAPI), and 4% paraformaldehyde (PFA), ascorbic acid (Thermo Fisher Scientific). .. Dexamethasone (Gibco, Grand Island, NY, USA), insulin–transferrin–selenium (ITS (1X), Sigma-Aldrich, Saint Louis, MO, USA), D1 mouse mesenchymal stem cells (ATCC, passage 11).

    Article Title: Atlas of human gut-associated lymphoid tissue reveals immunomodulatory interactions of B cells.
    Article Snippet: Gutassociated lymphoid tissue (GALT) is organized lymphoid tissue that responds chronically to antigens, including whole bacteria, sampled from the gut lumen.. The ensuing immunoglobulin A (IgA) plasma cell response disseminates to regulate bacterial populations and to mediate intestinal immune homeostasis.. GALT has roles in the development of the innatelike marginal zone B cell population and is associated with a B cell–mediated contribution to ulcerative colitis (UC) severity and response to therapy.

    Article Title: Organoid-based colorectal tumor microenvironment model for immuno-oncology research
    Article Snippet: Media, consisting of a 1:1 mixture of Advanced DMEM-F12 (12634-010, gibco) + GlutaMax-I (35050-038, gibco) + Penicilin/Streptomycin (P/S) (15140-122, gibco) and unsupplemented OGM (100-0190 StemCell), was exchanged every two days, except over the weekend when a three-day interval was permitted. .. For passaging, media was removed, and wells were washed with 500 μl of Dulbecco’s Phosphate Buffered Saline (DPBS) (14190-094, Gibco) and 500 μl of Gentle Cell Dissociation Reagent (100-0485, StemCell) was added to each dome. ..

    Article Title: Tuning the mechanical properties of polymer-based surrogate materials for articular cartilage and vocal fold repair
    Article Snippet: .. After about 60 seconds, the glue had cured and the previously attached immersion cup was filled with Dulbecco’s phosphate-buffered saline (DPBS) (Gibco Life Technologies, Thermo Fisher Scientific, Waltham, USA) until the entire specimen was covered in liquid. ..

    Article Title: Receptor-Tethered Cytosolic Modulators Enable Spatial Control of Cell Signaling Specificity.
    Article Snippet: Membrane receptors sense external stimuli and transduce signals by recruiting cytosolic effector proteins to initiate downstream signaling cascades.. Despite precise and distinct functional outcomes, diverse receptors often engage common cytosolic effectors, implying that signaling specificity is regulated by the spatial dynamics of effector activity within membrane-proximal cytoplasmic microdomains.. Here, we engineered receptor-tethered cytosolic modulators for manipulating the activity of effectors within localized cytoplasmic regions adjacent to target receptors.

    Article Title: Mtf2 Safeguards Naïve Pluripotency by Restricting Trophectoderm Competence
    Article Snippet: .. For single-cell RNA-sequencing, cells cultured in 60 mm dishes were washed with pre-warmed Dulbecco’s Phosphate-Buffered Saline (DPBS) (Gibco). .. Cells were then dissociated using Accutase (BD Biosciences) after a 3-minute incubation at 37°C.

    Modification:

    Article Title: Ice Templated PEG-Alginate Double-Network Cryogels with Tunable Mechanics and Degradation for Soft Tissue Engineering.
    Article Snippet: 8-arm polyethylene glycol acrylate (8-arm PEG acrylate, 10 kDa; Jenkem Technology, Plano, TX, USA), sodium alginate with an M/G ratio of 0.6 (Protanal® LF 10/60, IFF Pharma Solutions, Wilmington, DE, USA), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC; Thermo Fisher Scientific, Waltham, MA, USA), N-hydroxysuccinimide (NHS; Tokyo Chemical Industry, Tokyo, Japan), 2-(N-morpholino) ethanesulfonic acid buffer (MES, 1.0 M, pH 7.0; Thermo Fisher Scientific), and adipic acid dihydrazide (AAD; Tokyo Chemical Industry). .. Dithiothreitol (DTT) and ethylene glycol bis(mercaptoacetate) (EGBMA), Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12, 1:1), fetal bovine serum (FBS), penicillin–streptomycin (PS), L-glutamine, Dulbecco’s phosphate-buffered saline (DPBS), and 0.05% trypsin–EDTA, Rat tail collagen type I, The LIVE/DEAD Viability/Cytotoxicity Kit, 4′,6-diamidino-2-phenylindole (DAPI), and 4% paraformaldehyde (PFA), ascorbic acid (Thermo Fisher Scientific). .. Dexamethasone (Gibco, Grand Island, NY, USA), insulin–transferrin–selenium (ITS (1X), Sigma-Aldrich, Saint Louis, MO, USA), D1 mouse mesenchymal stem cells (ATCC, passage 11).

    Article Title: Receptor-Tethered Cytosolic Modulators Enable Spatial Control of Cell Signaling Specificity.
    Article Snippet: Membrane receptors sense external stimuli and transduce signals by recruiting cytosolic effector proteins to initiate downstream signaling cascades.. Despite precise and distinct functional outcomes, diverse receptors often engage common cytosolic effectors, implying that signaling specificity is regulated by the spatial dynamics of effector activity within membrane-proximal cytoplasmic microdomains.. Here, we engineered receptor-tethered cytosolic modulators for manipulating the activity of effectors within localized cytoplasmic regions adjacent to target receptors.

    Cell Counting:

    Article Title: Atlas of human gut-associated lymphoid tissue reveals immunomodulatory interactions of B cells.
    Article Snippet: Gutassociated lymphoid tissue (GALT) is organized lymphoid tissue that responds chronically to antigens, including whole bacteria, sampled from the gut lumen.. The ensuing immunoglobulin A (IgA) plasma cell response disseminates to regulate bacterial populations and to mediate intestinal immune homeostasis.. GALT has roles in the development of the innatelike marginal zone B cell population and is associated with a B cell–mediated contribution to ulcerative colitis (UC) severity and response to therapy.

    FACS:

    Article Title: Atlas of human gut-associated lymphoid tissue reveals immunomodulatory interactions of B cells.
    Article Snippet: Gutassociated lymphoid tissue (GALT) is organized lymphoid tissue that responds chronically to antigens, including whole bacteria, sampled from the gut lumen.. The ensuing immunoglobulin A (IgA) plasma cell response disseminates to regulate bacterial populations and to mediate intestinal immune homeostasis.. GALT has roles in the development of the innatelike marginal zone B cell population and is associated with a B cell–mediated contribution to ulcerative colitis (UC) severity and response to therapy.

    Passaging:

    Article Title: Organoid-based colorectal tumor microenvironment model for immuno-oncology research
    Article Snippet: Media, consisting of a 1:1 mixture of Advanced DMEM-F12 (12634-010, gibco) + GlutaMax-I (35050-038, gibco) + Penicilin/Streptomycin (P/S) (15140-122, gibco) and unsupplemented OGM (100-0190 StemCell), was exchanged every two days, except over the weekend when a three-day interval was permitted. .. For passaging, media was removed, and wells were washed with 500 μl of Dulbecco’s Phosphate Buffered Saline (DPBS) (14190-094, Gibco) and 500 μl of Gentle Cell Dissociation Reagent (100-0485, StemCell) was added to each dome. ..

    Gentle:

    Article Title: Organoid-based colorectal tumor microenvironment model for immuno-oncology research
    Article Snippet: Media, consisting of a 1:1 mixture of Advanced DMEM-F12 (12634-010, gibco) + GlutaMax-I (35050-038, gibco) + Penicilin/Streptomycin (P/S) (15140-122, gibco) and unsupplemented OGM (100-0190 StemCell), was exchanged every two days, except over the weekend when a three-day interval was permitted. .. For passaging, media was removed, and wells were washed with 500 μl of Dulbecco’s Phosphate Buffered Saline (DPBS) (14190-094, Gibco) and 500 μl of Gentle Cell Dissociation Reagent (100-0485, StemCell) was added to each dome. ..

    Single Cell:

    Article Title: Mtf2 Safeguards Naïve Pluripotency by Restricting Trophectoderm Competence
    Article Snippet: .. For single-cell RNA-sequencing, cells cultured in 60 mm dishes were washed with pre-warmed Dulbecco’s Phosphate-Buffered Saline (DPBS) (Gibco). .. Cells were then dissociated using Accutase (BD Biosciences) after a 3-minute incubation at 37°C.

    RNA sequencing:

    Article Title: Mtf2 Safeguards Naïve Pluripotency by Restricting Trophectoderm Competence
    Article Snippet: .. For single-cell RNA-sequencing, cells cultured in 60 mm dishes were washed with pre-warmed Dulbecco’s Phosphate-Buffered Saline (DPBS) (Gibco). .. Cells were then dissociated using Accutase (BD Biosciences) after a 3-minute incubation at 37°C.

    Cell Culture:

    Article Title: Mtf2 Safeguards Naïve Pluripotency by Restricting Trophectoderm Competence
    Article Snippet: .. For single-cell RNA-sequencing, cells cultured in 60 mm dishes were washed with pre-warmed Dulbecco’s Phosphate-Buffered Saline (DPBS) (Gibco). .. Cells were then dissociated using Accutase (BD Biosciences) after a 3-minute incubation at 37°C.



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